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Activity of methanogens in biomethanation digester is totally inhibited due to :

  • A.More oxygen (8 ppm)
  • B.Neutral pH (6.8-7.2) of feedstock slurry
  • C.Presence of syntrophic microbes
  • D.Absence of oxygen

Answer: A

Methanogens are inhibited by more oxygen (8 ppm).

Methanogens are strict anaerobes: they lack enzymes such as catalase and superoxide dismutase to cope with oxygen, and key enzymes of methanogenesis are destroyed by it. Dissolved oxygen around 8 ppm, close to saturation in water, therefore stops methane production completely.

A neutral pH of 6.8-7.2 is the optimum range for methanogens, syntrophic bacteria help them by supplying hydrogen and acetate, and the absence of oxygen is exactly the condition they need. None of these inhibits them.

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