Discussion
Match the entries in Group I with that in Group II
| Group I | Group II |
|---|---|
| P) Fehling's test | 1) α-Amino acid |
| Q) Ninhydrin reaction | 2) Reducing sugar |
| R) Biuret reaction | 3) Sulfhydryl group |
| S) Nitroprusside reaction | 4) Peptide linkage |
Answer: C
The correct match is P-2, Q-1, R-4, S-3.
- Fehling's test detects reducing sugars, which reduce Cu2+ to red Cu2O.
- Ninhydrin reacts with the free amino group of α-amino acids to give a purple colour.
- The biuret reaction detects peptide linkages, giving a violet colour with Cu2+ in alkali.
- The nitroprusside reaction detects free sulfhydryl (-SH) groups, as in cysteine.
The option P-4, Q-1, R-2, S-3 gets ninhydrin and nitroprusside right but swaps Fehling's and biuret. The other two misplace ninhydrin as well.
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