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If cells of Escherichia coli are grown in a medium containing radioactive 32P, the 32P would be found in all, except :

  • A.DNA
  • B.RNA
  • C.ATP
  • D.Carbohydrates

Answer: D

The 32P would not be found in carbohydrates.

DNA and RNA contain phosphate in every nucleotide of their sugar-phosphate backbone, and ATP carries three phosphate groups, so all three take up labelled phosphorus. This is why Hershey and Chase used 32P to label phage DNA.

Carbohydrates as such are made of carbon, hydrogen and oxygen only. Phosphorylated sugars occur as metabolic intermediates, but the carbohydrates of the cell, such as glycogen and the polysaccharides, contain no phosphorus, so they are the exception.

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