Discussion
For brucellosis, 2 ME agglutination test is used to identify
Answer: A
Correct answer: IgG
Mercaptoethanol breaks the disulphide bonds holding the IgM pentamer together, destroying its agglutinating activity while leaving IgG unaffected. Agglutination that survives treatment is therefore due to IgG. That matters clinically: IgM appears early and may persist after cure, whereas continuing IgG indicates chronic or active infection rather than a past exposure.
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