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Adherent cells are usually detached from a culture vessel using

  • A.ethanol, which dissolves the cell membranes
  • B.strong acid, which dissolves the plastic vessel
  • C.trypsin, which digests the attachment proteins
  • D.distilled water, which bursts the cells open

Answer: C

The enzyme cleaves the proteins anchoring the cells to the surface and to one another. Exposure must be brief, since prolonged treatment damages surface proteins the cells need. Serum-containing medium is added afterwards to neutralise the enzyme.

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