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Culture medium for animal cells typically contains

  • A.amino acids, vitamins, salts and glucose
  • B.only distilled water together with glucose
  • C.agar and a very small amount of salt
  • D.nothing except a buffered saline solution

Answer: A

Serum is usually added as well, supplying growth factors and attachment proteins. Its composition is poorly defined, which is why serum-free formulations are preferred for reproducible work. Defined media remove one uncontrolled variable from every experiment.

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