Discussion
A carbon dioxide incubator is used because the medium is buffered by
Answer: D
Correct answer: a bicarbonate system needing carbon dioxide
Most media are buffered by bicarbonate, and that buffer only holds its pH in equilibrium with a defined partial pressure of carbon dioxide - typically five per cent. In ordinary air the dissolved carbon dioxide escapes and the medium turns alkaline, which the phenol red indicator shows as a shift towards purple. Media buffered with HEPES instead can be handled outside an incubator for the same reason.
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