Discussion
Assertion (A): Laboratories test their cultures for mycoplasma routinely rather than waiting for signs of trouble.
Reason (R): Mycoplasma causes no visible change in the appearance of a culture.
Answer: D
Correct answer: Both A and R are true, and R is the correct explanation of A
Both are true and the reason gives the necessity. Mycoplasma are too small to see by light microscopy, do not cloud the medium and do not kill the cells, so an infected culture looks entirely normal - while its metabolism, gene expression and drug responses are all altered. Nothing about routine observation would reveal it, so periodic testing by PCR or staining is the only defence.
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