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Confluence in a culture of adherent cells describes

  • A.the proportion of the surface covered by cells
  • B.the number of times the culture has been passaged
  • C.the concentration of serum in the medium
  • D.the temperature at which cells are grown

Answer: A

Cells are usually passaged before they reach full coverage, since crowding slows growth and can change their behaviour. It is judged by eye under a microscope rather than measured. Judging it consistently takes a little practice.

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