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Cells are frozen for long-term storage in the presence of a cryoprotectant such as DMSO in order to

  • A.keep the cells dividing while frozen
  • B.sterilise the sample before it is stored
  • C.prevent ice crystals damaging the cells
  • D.supply the cells with additional nutrients

Answer: C

Ice crystals forming inside a cell tear its membranes apart, and the cryoprotectant limits their growth. Cooling slowly and thawing quickly are both part of the same strategy. Both rates are chosen to give ice as little chance to form as possible.

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