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Trypan blue is used in cell counting because it

  • A.makes the cells fluoresce under ultraviolet light
  • B.enters dead cells but is excluded by living ones
  • C.stains every cell in the sample equally
  • D.kills any bacteria present in the sample

Answer: B

An intact membrane keeps the dye out, so blue cells are dead and clear ones alive. The test measures membrane integrity rather than any deeper notion of viability. A cell can be dying and still exclude the dye for some hours.

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