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State whether the following is true or false.
A cell that excludes trypan blue is certainly healthy.

  • A.False only at small scales
  • B.True
  • C.False
  • D.True only at large scales

Answer: C

Exclusion shows only that the membrane is intact, and a cell can be committed to apoptosis while still excluding the dye. The test is a useful screen rather than a verdict on the state of the cell. Apoptotic cells commonly exclude it until quite late in the process.

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