Discussion
Growing cells in three dimensions rather than as a flat layer is valuable because it
Answer: B
Correct answer: better reproduces conditions inside a tissue
Cells in a flat layer sit on plastic, receive nutrients uniformly and have neighbours on one side only. In three dimensions they contact each other and a matrix on all sides, and gradients of oxygen, nutrients and waste develop as they do in tissue. Gene expression, differentiation and drug response all shift accordingly, which is why a compound can behave quite differently in the two systems.
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