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Antibiotics are sometimes added to culture medium, but their routine use is discouraged because they

  • A.mask poor technique and hide low-level contamination
  • B.are toxic to every single kind of cultured cell
  • C.prevent the cells from attaching to the culture vessel
  • D.make the medium change its colour quite unpredictably

Answer: A

A contamination held in check rather than eliminated persists undetected and may flare later. Good aseptic technique is a better safeguard than a routine additive. Antibiotics also fail entirely against fungal and mycoplasma contamination.

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