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The main practical advantage of an immortalised line over a primary culture is that it

  • A.behaves in exactly the same way as the original tissue
  • B.requires neither medium nor an incubator in order to grow
  • C.cannot ever become contaminated by bacteria or fungi
  • D.provides an unlimited and consistent supply of cells

Answer: D

Correct answer: provides an unlimited and consistent supply of cells

An immortalised line divides indefinitely and can be expanded, frozen and shared, so experiments can be repeated by anyone with the same starting material. What is given up is fidelity: immortalisation and long culture change the cells, so they no longer behave exactly like the tissue they came from. That is the trade-off the next item refers to.

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