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Stem cells are cultured under conditions that must

  • A.keep them entirely free of any growth factor
  • B.maintain them without letting them differentiate
  • C.force them to differentiate as fast as possible
  • D.prevent them from dividing at any point

Answer: B

Specific factors hold the cells in an undifferentiated state, and withdrawing or changing them directs the cells down a chosen path. Getting that balance right is much of the difficulty of the work. Small changes in the medium can send the cells down the wrong path.

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