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Conditioned medium is medium that has

  • A.had every trace of its serum removed by filtration
  • B.been stored for considerably longer than its shelf life
  • C.already been used by cells and retains their secretions
  • D.been sterilised at a high temperature in an autoclave

Answer: C

The factors secreted into it can support other cultures, which is a simple way to supply signals that are difficult to identify individually. It shares the drawback of serum in being undefined. Its usefulness lies in supplying factors nobody has yet identified.

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