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Cryopreservation is a process of :

  • A.ex situ in vitro conservation
  • B.ex situ ex vitro conservation
  • C.in situ in vitro conservation
  • D.in situ ex vitro conservation

Answer: A

Cryopreservation is ex situ in vitro conservation.

In cryopreservation, seeds, embryos, pollen, shoot tips or cell cultures are stored at very low temperature, usually in liquid nitrogen at −196°C, where metabolism effectively stops. It takes material away from its natural habitat, so it is ex situ, and it keeps tissues and cells in culture vessels and vials rather than as whole growing plants, so it is in vitro.

Ex situ ex vitro conservation means botanical gardens, field gene banks and zoos. In situ conservation keeps organisms in their habitats, such as national parks and biosphere reserves, and is never in vitro.

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