Discussion
Which of the following is used for the proper maintenance and preservation of pure cultures?
Answer: D
Correct answer: All of the above
All three are standard. Periodic transfer to fresh medium keeps a culture alive but risks contamination and mutation over many passages. Overlaying with mineral oil slows drying and metabolism, extending the interval between transfers. Lyophilisation - freeze drying - is the best for long-term storage, keeping cultures viable for years with no change.
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