Discussion
For the selection of endospore-forming bacteria, a mixed culture can be heated at
Answer: A
Correct answer: 80°C for 10 minutes
Heating a mixed culture to 80°C for 10 minutes kills all the vegetative cells while endospores survive, since they resist heat far better. Subculturing afterwards therefore yields only spore-formers - Bacillus and Clostridium. It is selection by physical means rather than chemical.
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