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The concentration of agar to obtain semi solid media is

  • A.1.5-20%
  • B.0.5% or less
  • C.>10%
  • D.>10%but<20%

Answer: B

Correct answer: 0.5% or less

Semi-solid medium uses about 0.5% agar or less, giving a soft gel through which motile organisms can swim - which is how motility is tested, by whether growth spreads out from the stab line. A firm plate needs 1.5-2%.

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