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In the pour plate method, the mixed culture is diluted directly in tubes of

  • A.liquid agar medium
  • B.sterile liquid usually water
  • C.both (a) and (b)
  • D.none of these

Answer: A

Correct answer: liquid agar medium

In the pour plate method the sample is mixed into molten agar cooled to about 45°C, and the mixture is poured into the dish. Colonies therefore develop within the agar as well as on it, which suits microaerophiles and gives good separation - at the cost of subjecting the cells to that brief warmth.

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