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A high dissolved oxygen concentration in the culture medium is toxic and leads to

  • A.DNA degradation
  • B.lipid peroxidation
  • C.metabolism of nutrients in culture media at a rate greater than that required for consumption
  • D.all of the above

Answer: D

Correct answer: all of the above

Too much dissolved oxygen generates reactive oxygen species, and all three consequences follow: DNA damage, lipid peroxidation of the membranes, and excessive metabolism of the medium’s nutrients. This is why oxygen is controlled rather than simply maximised in a bioreactor.

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