Discussion
An established cell line can be called where it has been subcultured at least
Answer: A
Correct answer: 70 times at an interval of 3 days between subcultures
The working definition of an established line is one subcultured about 70 times at three-day intervals - long past the fifty or so divisions at which a normal human cell reaches senescence. Surviving that many passages is the practical evidence that the line has become continuous.
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