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During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

  • A.Ethyl alcohol is being produced in excess
  • B.The cells have too much oxygen
  • C.Glycolysis is being inhibited
  • D.The cells do not have enough oxygen

Answer: D

Correct answer: The cells do not have enough oxygen

Lactate accumulates when cells fall back on anaerobic glycolysis, and that happens when the oxygen supply is inadequate for oxidative phosphorylation. The lactate then acidifies the medium, which damages the cells further. The remedy is better aeration or a lower cell density.

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