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Range of optimum glucose concentration present in the culture media is

  • A.5.5 - 55 mmol/litre
  • B.55 - 75 mmol/litre
  • C.75-105 mmol/litre
  • D.105-150 mmol/litre

Answer: A

Correct answer: 5.5 – 55 mmol/litre

Culture media hold glucose at about 5.5 to 55 mmol/litre - the lower end matching blood glucose, the higher end used where cells are to be grown to high density. Too much is not simply wasteful: excess glucose drives fermentation to lactate even in air, and the acid that results limits growth.

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