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Which of the following is correct?

  • A.ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
  • B.ECL and PCL can be established in suspension cultures
  • C.PCL can be established in suspension cultures
  • D.none of the above

Answer: A

Correct answer: ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)

Established lines are commonly anchorage independent, so they can be grown in suspension - which is what makes large-scale culture in stirred bioreactors possible. Primary lines are almost always anchorage dependent and must be grown on a surface, so suspension culture is exceptional for them.

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