Discussion
To prevent the accumulation of lactate
Answer: C
Correct answer: low glucose concentration is required
Lactate comes from glucose, and cells ferment more of it the more there is - even when oxygen is plentiful. Keeping the glucose concentration low, by feeding it gradually rather than supplying it all at once, forces the cells to oxidise what they get and so limits lactate. That is the principle of fed-batch culture.
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