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Discussion

According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

  • A.6 amino acids
  • B.8 amino acids
  • C.10 amino acids
  • D.12 amino acids

Answer: D

Correct answer: 12 amino acids

Eagle showed that L-strain and HeLa cells require twelve amino acids in the medium - the nine essential for humans plus cysteine, tyrosine and glutamine, which cultured cells cannot make in sufficient quantity. His systematic omission experiments are the foundation of every defined medium since.

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