Discussion
Toxicity due to the accumulation of ammonia can be overcome
Answer: D
Correct answer: all of the above
All three help. Substituting glutamate for glutamine removes the amide nitrogen that becomes ammonia; feeding glutamine slowly keeps its concentration low, so less is deaminated; and removing the ammonium as it forms - by dialysis or by ion exchange - deals with what is produced. All are used in high-density culture.
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