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The material used in culture techniques is important because the surface of the vessel serves as a substrate for the cells to grow on.

  • A.True 
  • B.False  

Answer: A

Correct answer: True

Most animal cells are anchorage dependent, so the surface itself is part of the culture system. Ordinary plastic is too hydrophobic, which is why culture vessels are surface treated to carry a charge, and why coatings of collagen, fibronectin or poly-lysine are used for cells that attach poorly.

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