Discussion
What is the preparation of Nutrient Agar (NA) in common culture media?
Answer: A
Nutrient agar is made with 0.5% peptone, 0.3% beef extract and 1.5% agar.
Nutrient agar is nutrient broth (peptone 0.5%, beef extract 0.3% and usually sodium chloride 0.5%) solidified with about 1.5% agar. Peptone and beef extract supply nitrogen, vitamins and minerals; agar is only a solidifying agent that bacteria do not digest.
Peptone water is 1% peptone with 0.5% NaCl, a liquid medium with no agar, which is the second option. The other two mixtures, with 2% or 3% peptone or 2% salt, do not correspond to the standard formula.
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