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Density-dependent inhibition of growth in culture refers to cells

  • A.requiring no growth factors when crowded
  • B.ceasing division once a monolayer forms
  • C.dividing more quickly as they become crowded
  • D.detaching from the dish as they multiply

Answer: B

Nutrients and growth factors become limiting and cell-to-cell contact signals arrest, so growth stops at a characteristic density. Transformed cells ignore both signals and keep going. Piling up into disordered heaps is a standard sign of transformation in culture.

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