Discussion
The resolution limit of a light microscope is set principally by
Answer: B
Correct answer: the wavelength of the light used
Resolution is bounded by diffraction: \[ d = \frac{0.61\lambda}{\mathrm{NA}}, \] so with visible light around 500 nm and the best objectives, about 200 nm is the limit. Brighter illumination and stronger eyepieces enlarge the image without separating anything further, which is exactly the distinction between magnification and resolution.
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