Discussion
Consider the following statements about microscopy.
1. Resolution is limited by the wavelength of the illumination.
2. Electron microscopy generally requires the specimen to be dead.
3. Magnification and resolution mean the same thing.
Answer: B
Correct answer: 1 and 2 only
Statements 1 and 2 are correct: resolution is set by the wavelength, and electron microscopy requires a high vacuum along with fixing, dehydration and often metal coating, so the specimen cannot be alive. Statement 3 is the confusion this topic keeps returning to - magnification is how much larger the image is, resolution is how close two points can be and still be seen as two, and enlarging an image beyond its resolution adds nothing.
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