Discussion
What is the sequence of cell organelles getting separated during cell fractionation by differential centrifugation ?
Answer: A
The order is nucleus, mitochondria, microsomes, cytosol.
In differential centrifugation a homogenate is spun at increasing speeds, and the largest, densest particles sediment first. Nuclei come down at low speed (about 600-1,000 g), mitochondria (with lysosomes and peroxisomes) at medium speed (about 10,000-20,000 g), and microsomes, the fragments of ER, at high speed (about 100,000 g). The supernatant left at the end is the cytosol.
Every other option puts the cytosol or the microsomes too early; the cytosol never forms a pellet, so it is always last.
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