Discussion
Consider the following statements about binary vector used for plant transformation ?
(i) Binary vector are Agrobacterium based vectors
(ii) Binary vector contains both bacterial and plant selectable marker
(iii) Binary vector have virulence (Vir) region
(iv) There is presence of T-DNA left and right border repeat sequences
Which of the above statements are correct :
Answer: C
Statements (i), (ii) and (iv) are correct.
A binary vector system splits the Ti plasmid's functions between two plasmids in Agrobacterium (i). The small binary vector carries the T-DNA left and right border repeats (iv), with the gene of interest and a plant selectable marker between them, and a bacterial selectable marker and origins outside the borders for growth in E. coli and Agrobacterium (ii).
Statement (iii) is false. The virulence (vir) genes are on the second, disarmed helper Ti plasmid, which acts in trans to transfer the T-DNA. That is the point of the binary design, so every option including (iii) is wrong.
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