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Which of the following is NOT correct for an ideal plasmid cloning vector ?

  • A.Smaller size
  • B.Presence of multiple cloning site
  • C.Absence of origin of replication
  • D.Presence of selectable marker

Answer: C

The feature that is not correct is the absence of an origin of replication.

A plasmid vector must replicate in the host cell to be maintained and multiplied, so an origin of replication is essential. Without one the vector and its insert would be lost as the cells divide.

The other features are desirable. A small size makes the plasmid easy to handle, gives a high copy number and leaves room for inserts. A multiple cloning site offers unique restriction sites for inserting DNA, and a selectable marker such as antibiotic resistance identifies cells that carry the vector.

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