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Two micron plasmid can be obtained from the following yeast :

  • A.Candida shehatae
  • B.Pichia pastoris
  • C.Schizosaccharomyces pombe
  • D.Saccharomyces cerevisiae

Answer: D

The two micron plasmid is obtained from Saccharomyces cerevisiae.

The 2-micron (2 µm) plasmid is a circular DNA of about 6.3 kb, named for its contour length, present in some 40-60 copies per cell in most strains of baker's yeast. Its origin of replication is used to build high-copy yeast episomal plasmid vectors (YEp).

Candida shehatae, Pichia pastoris and the fission yeast Schizosaccharomyces pombe do not carry the 2-micron plasmid.

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