Discussion
Which of the following is a phagemid vector ?
Answer: D
The phagemid vector is pBluescript.
A phagemid is a plasmid that also carries the origin of replication of a filamentous phage such as f1 or M13. pBluescript has the f1 origin, so in cells infected with a helper phage it can be packaged as single-stranded DNA, useful for sequencing and mutagenesis, while it otherwise replicates as an ordinary plasmid.
pUC19 and pBR322 are plain plasmid vectors with no phage origin, and λEMBL3 is a lambda replacement vector, a phage rather than a phagemid.
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