Discussion
Nagler’s reaction is shown by:
Answer: C
Correct answer: Cl. Perfringens
Nagler’s reaction demonstrates the lecithinase activity of alpha toxin. C. perfringens is grown on egg yolk agar half of which carries antitoxin: opalescence appears around the growth on the untreated half where the lecithin has been split, and is absent where antitoxin has neutralised the enzyme. It is the specific inhibition by antitoxin that makes the test conclusive.
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