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The growth of bacteria (lactobacillus) in milk leads to curd formation. A minimum bacterial population density of 0.8 (in suitable units) is needed to form curd. In the graph below, the population density of lactobacillus in 1 litre of milk is plotted as a function of time, at two different temperatures, 25 °C and 37 °C.

Population density against time from 0 to 200 min: the 37 C curve reaches 0.8 at about 85 min and levels off near 1.0 from about 150 min; the 25 C curve reaches 0.8 at about 125 min and levels off near 1.0 from about 180 min

Consider the following statements based on the data shown above:

i. The growth in bacterial population stops earlier at 37 °C as compared to 25 °C
ii. The time taken for curd formation at 25 °C is twice the time taken at 37 °C

Which one of the following options is correct?

  • A.Only i
  • B.Only ii
  • C.Both i and ii
  • D.Neither i nor ii

Answer: A

The correct option is Only i.

Statement i: the 37 °C curve levels off near 1.0 by about 150 min, while the 25 °C curve keeps rising until about 180 to 190 min. So growth stops earlier at 37 °C, and statement i is true.

Statement ii: curd forms when the density reaches 0.8. The 37 °C curve crosses 0.8 at about 85 min and the 25 °C curve at about 125 min. 125 is roughly 1.5 times 85, not twice, so statement ii is false.

That rules out "Only ii" and "Both i and ii", and "Neither" ignores the earlier plateau at 37 °C.

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