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Which enzyme is used to join bits of DNA?

  • A.DNA polymerase
  • B.DNA ligase
  • C.Endonuclease
  • D.Primase

Answer: B

Correct answer: DNA ligase

DNA ligase forms the phosphodiester bond that closes a nick between two adjacent fragments. It is what joins Okazaki fragments in the cell, and what joins an insert to a vector in cloning. Polymerase extends a chain from a primer; endonucleases and primases cut and prime respectively.

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