Discussion
Which of the following would relax negatively supercoiled DNA?
Answer: B
Correct answer: Type I topoisomerase
Type I topoisomerase nicks one strand, lets the DNA swivel to release one supercoil, and reseals - needing no ATP, because relaxing a supercoil is energetically downhill. DNA gyrase does the opposite: it uses ATP to introduce negative supercoils. Collagen and elastin are structural proteins with no role here.
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