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Discussion

DNA helicase is used to

  • A.unwind the double helix
  • B.interact the double helix closely
  • C.break a phosphodiester bond in DNA strand
  • D.none of the above

Answer: A

Correct answer: unwind the double helix

Helicase separates the two strands by breaking the hydrogen bonds between the paired bases, using the energy of ATP. It does not touch the covalent phosphodiester backbone - cutting that is the work of nucleases and topoisomerases.

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