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Discussion

DNA can be sequenced by the

  • A.chemical method
  • B.chain termination procedure
  • C.both (a) and (b)
  • D.physical method

Answer: C

Correct answer: both (a) and (b)

Two classical methods: the Maxam-Gilbert chemical method, which cleaves end-labelled DNA at specific bases, and Sanger’s chain-termination method, which uses dideoxynucleotides to stop synthesis at each position. Sanger sequencing proved far easier to automate and became the standard.

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