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The deoxyribonucleoside triphosphates needed in the chain-termination method of sequencing are

  • A.dATP
  • B.dGTP and dCTP
  • C.dTTP
  • D.all of these

Answer: D

Correct answer: all of these

Sanger sequencing needs all four normal deoxyribonucleoside triphosphates - dATP, dGTP, dCTP and dTTP - so that the chain can be extended, plus a small proportion of dideoxynucleotides to terminate it at random positions. Leaving any of the four out would stop synthesis wherever that base was required.

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