Discussion
Overwinding or overtightening of DNA during replication is caused and removed by
Answer: C
Correct answer: DnaB, gyrase
DnaB is the replicative helicase: by unwinding the duplex at the fork it drives the DNA ahead of it into ever tighter positive supercoils - the overwinding. Gyrase then removes that strain, cutting both strands, passing the duplex through and resealing, so the fork can keep moving.
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