Discussion
How does ethidium bromide interact with DNA?
Answer: C
Correct answer: It intercalates between bases
Ethidium bromide is a flat aromatic cation that slides between adjacent base pairs, into the stack. Its fluorescence rises sharply once it is in that hydrophobic environment, which is what makes DNA bands glow under UV. Intercalation also unwinds and lengthens the helix slightly - and, by distorting it, makes the dye a mutagen, which is why it is handled with care.
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