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Replication in E. coli is initiated by the generation of short RNA primers using

  • A.RNA polymerase
  • B.primase
  • C.reverse transcriptase
  • D.both (a) and (b)

Answer: D

Correct answer: both (a) and (b)

Primase (the DnaG protein) makes the primers at the fork, once on the leading strand and once for each Okazaki fragment. RNA polymerase also has a part at initiation: its transcription across the origin region helps open the duplex, and in some replicons it makes the primer itself. So both enzymes are involved in generating the RNA that starts replication.

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